Myrcene exerts anti-tumor effects on oral cancer cells in vitro via induction of apoptosis

Purpose: To investigate the anticancer potential of myrcene against oral cancer cells.Methods: The effect on cell growth were studied using CCK-8 proliferation andclonogenic assays, while Annexin V-FITC/PI and DAPI staining methods used for determination its apoptosis. Protein expression levels assayed by western blotting technique. Wound healing Transwell chamber assays to determine migration invasion, respectively.Results: Myrcene significantly dose-dependently inhibited (p < 0.05). anti-proliferative was comparatively lower Hs27 human fibroblast cells than SCC9 cells. stimulated apoptosis in via enhancement Bax repression Bcl-2 expression. percentage early late apoptotic increased from 4.03 untreated control 27.77 when treated with 20 μM myrcene. Moreover, vitro suppressed invasion 0.05).Conclusion: These results indicate that exerts potent effects induction apoptosis, should be further investigated vivo this purpose.